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Articles
Published: 26-12-2022

Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article

FACERES – Faculty of Medicine of Sao Jose do Rio Preto, Sao Paulo, Brazil / ABRAN – Associação Brasileira de Nutrologia/Brazilian Association of Nutrology, Catanduva, Sao Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Unioftal – Ophthalmology And Eye Plastic, São José do Rio Preto, São Paulo, Brazil
Adipose tissue Stromal vascular fraction Mechanical technique Optimization

Abstract

The present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter. It was observed that the technique of the present study obtained a characterization yield of the stromal vascular fraction of the adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.

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How to Cite

Zotarelli Filho, I. J., Liedtke, F. S., Ferrari, R. S., Daher, I. D., Neiva, E. B., Moura, M. I. P., & Murad, A. C. S. (2022). Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article. MedNEXT Journal of Medical and Health Sciences, 3(4). https://doi.org/10.54448/mdnt22412